ジャーナル
/
生物学
/
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

2,816 Views

•

09:21 min

•

November 30, 2021

DOI:

10.3791/63134-v

DOI:
10.3791/63134-v

•

09:21 min
November 30, 2021

•

8 Views
, , , , , , , , , ,
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience

概要

Automatically generated

This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.

Explore More Videos

Blood-brain BarrierIn Vitro ModelCell Culture SystemAstrocytesCell-cell CommunicationDrug DeliveryPreclinical AssessmentPoly-L-LysineTrypsinAstrocyte MediumCollagen Type 1

Read Article

Cite this Article

Rizzi, E., Deligne, C., Dehouck, L., Bilardo, R., Sano, Y., Shimizu, F., Kanda, T., Resmini, M., Gosselet, F., Dehouck, M., Mysiorek, C. A Triple Culture Cell System Modeling the Human Blood-Brain Barrier. J. Vis. Exp. (177), e63134, doi:10.3791/63134 (2021).

Download .ris file

Copy

Share Video

.

Copy