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A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
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生物学
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JoVE Journal 生物学
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

2,785 Views

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09:21 min

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November 30, 2021

DOI:

10.3791/63134-v

DOI:
10.3791/63134-v

•

09:21 min
November 30, 2021

•

8 Views
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1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience

概要

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This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.

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Cite this Article

Rizzi, E., Deligne, C., Dehouck, L., Bilardo, R., Sano, Y., Shimizu, F., Kanda, T., Resmini, M., Gosselet, F., Dehouck, M., Mysiorek, C. A Triple Culture Cell System Modeling the Human Blood-Brain Barrier. J. Vis. Exp. (177), e63134, doi:10.3791/63134 (2021).

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