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Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
Bölümler
- 00:05Introduction
- 01:15Bioluminescence Monitoring K18 hACE2 Transgenic Mice Infected with rSARS-CoV-2/Nluc
- 02:24Fluorescence Analysis in K18 hACE2 Transgenic Mice Infected with rSARS-CoV-2/Venus
- 03:49Viral Titrations
- 05:48Results: rSARS-CoV-2Nluc and rSARS-CoV-2/Venus Expression in Infected K18 Hace2 Transgenic Mice
- 08:12Conclusion
This protocol describes the dynamics of viral infections using luciferase- and fluorescence-expressing recombinant (r)SARS-CoV-2 and an in vivo imaging systems (IVIS) in K18 hACE2 transgenic mice to overcome the need of secondary approaches required to study SARS-CoV-2 infections in vivo.
