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Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex
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JoVE Journal Nörobilim
Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

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11:31 min

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February 25, 2022

DOI:

10.3791/63077-v

DOI:
10.3791/63077-v

•

11:31 min
February 25, 2022

•

2 Views
,
1School of Physiology, Pharmacology and Neuroscience,University of Bristol

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Here we present a protocol describing viral transduction of discrete brain regions with optogenetic constructs to permit synapse-specific electrophysiological characterization in acute rodent brain slices.

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OptogeneticsSynaptic TransmissionSynaptic PlasticityMedial Prefrontal CortexLateral Entorhinal CortexVirally Delivered ConstructsAcute Brain SlicesLong-range PathwaysSelective StimulationStereotaxic InjectionViral PreparationBrain DissectionSucrose Cutting Solution

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Kinnavane, L., Banks, P. J. Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex. J. Vis. Exp. (180), e63077, doi:10.3791/63077 (2022).

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