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Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

6,334 Views

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10:57 min

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April 22, 2022

DOI:

10.3791/63069-v

DOI:
10.3791/63069-v

•

10:57 min
April 22, 2022

•

11 Views
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1Department of Medical Microbiology and Infectious Diseases,University of Manitoba, 2National Microbiology Laboratory,Public Health Agency of Canada

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This protocol presents methodology to perform biofilm growth and biomass measurements using self-assembled deep well PCR-plate devices for high-throughput 96-well pegged lid static biofilm screening.

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Doucet, A. N., Slipski, C. J., Golding, G. R., Mulvey, M. R., Bay, D. C. Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices. J. Vis. Exp. (182), e63069, doi:10.3791/63069 (2022).

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