Dergi
/
Biyokimya
/
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

DOI:
10.3791/63046-v

•

07:59 min

•

August 19, 2021

•

1Department of Biochemistry & Nutrition,Des Moines University

Bölümler

  • 00:05Introduction
  • 00:27Determination of Glycogen Synthase Activity
  • 02:28Measurement of Released Glucose
  • 03:55Determination of Glycogen Branching
  • 05:34Results: Spectrophotometric Assays of Glycogen Synthase
  • 07:31Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Techniques to measure the activity of key enzymes of glycogen metabolism are presented, using a simple spectrophotometer operating in the visible range.

Etiketler

Glycogen MetabolismSpectrophotometric MethodsGlycogen Synthase ActivityUDP GlucoseNADHGlycogenGlucose-6-phosphate DehydrogenaseHexokinaseEukaryotic Metabolism

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Determination of the Glycogen Content in Cyanobacteria

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Read Article