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通过联合共聚焦和超分辨率显微镜表征小鼠的神经肌肉接头
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
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通过联合共聚焦和超分辨率显微镜表征小鼠的神经肌肉接头

2,930 Views

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11:03 min

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December 08, 2021

DOI:

10.3791/63032-v

DOI:
10.3791/63032-v

•

11:03 min
December 08, 2021

•

5 Views
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1Genethon, 91000, Evry, France, 2Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, 3Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France

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该协议描述了一种通过组合共聚焦和STED显微镜对神经肌肉接头进行形态学分析的方法，该方法用于量化SMA和ColQ相关CMS小鼠模型中的病理变化。

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Marinello, M., Cosette, J., Bogni, C., Denard, J., Stockholm, D., Buj-Bello, A. Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy. J. Vis. Exp. (178), e63032, doi:10.3791/63032 (2021).

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