Journal
/
神经科学
/
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

2,965 Views

•

11:03 min

•

December 08, 2021

DOI:

10.3791/63032-v

DOI:
10.3791/63032-v

•

11:03 min
December 08, 2021

•

5 Views
, , , , ,
1Genethon, 91000, Evry, France, 2Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, 3Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France

Summary

Automatically generated

This protocol describes a method for morphometric analysis of neuromuscular junctions by combined confocal and STED microscopy that is used to quantify pathological changes in mouse models of SMA and ColQ-related CMS.

Explore More Videos

Neuromuscular JunctionsConfocal MicroscopySuper-resolution MicroscopySTEDMuscle TeasingImmunostainingPresynaptic Axon TerminalsActive ZonesQuantitative Analysis3D StructureAnimal ModelsImage AcquisitionImage Analysis

Read Article

Cite this Article

Marinello, M., Cosette, J., Bogni, C., Denard, J., Stockholm, D., Buj-Bello, A. Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy. J. Vis. Exp. (178), e63032, doi:10.3791/63032 (2021).

Download .ris file

Copy

Share Video

.

Copy