/
/
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
4,706 Views
•
10:16 min
•
December 11, 2021
DOI:
1Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 4Department of Pathological Anatomy,North-Western State Medical University, 5Biyoloji Bölümü,Lomonosov State University
Özet
Automatically generated
Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.
Explore More Videos
Brain OrganoidInduced Pluripotent Stem CellsMini Bioreactors3D ModelCentral Nervous System PathologiesRegenerative MedicineDifferentiationShakerLow-adhesionBioreactorsNutrient MediumPlastic CrumbsChloroformLiquid PlasticPetri DishPluripotent Stem CellsDulbecco Modified Eagle MediumFisher-12 MediumSerum Replacement
Read Article
Cite this Article
Eremeev, A., Belikova, L., Ruchko, E., Volovikov, E., Zubkova, O., Emelin, A., Deev, R., Lebedeva, O., Bogomazova, A., Lagarkova, M. Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors. J. Vis. Exp. (178), e62987, doi:10.3791/62987 (2021).
Copy