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Reconstitución de la actividad de extracción de Msp1 con componentes totalmente purificados
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JoVE Journal Biyokimya
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
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Reconstitución de la actividad de extracción de Msp1 con componentes totalmente purificados

2,174 Views

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05:52 min

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August 10, 2021

DOI:

10.3791/62928-v

DOI:
10.3791/62928-v

•

05:52 min
August 10, 2021

•

6 Views
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1Department of Chemistry & Biochemistry,University of Toledo

Özet

Automatically generated

Aquí, presentamos un protocolo detallado para la reconstitución de la actividad de extracción de Msp1 con componentes totalmente purificados en proteoliposomas definidos.

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Msp1AAA-ATPaseProtein Quality ControlReconstitutionLipid BilayerTA ProteinsLiposomesBioBeadsGlutathione Spin ColumnsExtraction AssaySDS-PAGE

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Cite this Article

Fresenius, H. L., Wohlever, M. L. Reconstitution of Msp1 Extraction Activity with Fully Purified Components. J. Vis. Exp. (174), e62928, doi:10.3791/62928 (2021).

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