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Reconstitution of Msp1 Extraction Activity with Fully Purified Components
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JoVE Journal 生化学
Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

DOI:
10.3791/62928-v

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05:52 min

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August 10, 2021

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,
1Department of Chemistry & Biochemistry,University of Toledo

章

  • 00:05Introduction
  • 00:36Reconstitution of Msp1 and Model TA Protein
  • 02:37Extraction Assay
  • 04:47Results: Determining Extraction Efficiency
  • 05:26Conclusion

概要

自動翻訳

自動翻訳

Here, we present a detailed protocol for reconstitution of Msp1 extraction activity with fully purified components in defined proteoliposomes.

タグ

Msp1AAA-ATPaseProtein Quality ControlReconstitutionLipid BilayerTA ProteinsLiposomesBioBeadsGlutathione Spin ColumnsExtraction AssaySDS-PAGE

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