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用完全纯化的组分重构Msp1提取活性
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JoVE Journal Biyokimya
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
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用完全纯化的组分重构Msp1提取活性

DOI:
10.3791/62928-v

•

05:52 min

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August 10, 2021

•

,
1Department of Chemistry & Biochemistry,University of Toledo

Bölümler

  • 00:05Introduction
  • 00:36Reconstitution of Msp1 and Model TA Protein
  • 02:37Extraction Assay
  • 04:47Results: Determining Extraction Efficiency
  • 05:26Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里，我们提出了一个详细的方案，用于在定义的蛋白脂质体中用完全纯化的组分重建Msp1提取活性。

Etiketler

Msp1AAA-ATPaseProtein Quality ControlReconstitutionLipid BilayerTA ProteinsLiposomesBioBeadsGlutathione Spin ColumnsExtraction AssaySDS-PAGE

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