Despite recent advances, many yeast mitochondrial proteins still remain with their functions completely unknown. This protocol provides a simple and reliable method to determine the submitochondrial localization of proteins, which has been fundamental for the elucidation of their molecular functions.
Gomes, F., Turano, H., Ramos, A., Netto, L. E. S. Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae. J. Vis. Exp. (173), e62853, doi:10.3791/62853 (2021).