This protocol presents a collection of sarcomere, calcium, and macroscopic geometric data from an actively contracting cardiac trabecula ex vivo. These simultaneous measurements are made possible by the integration of three imaging modalities.
Dowrick, J. M., Anderson, A. J., Cheuk, M. L., Tran, K., Nielsen, P. M. F., Han, J., Taberner, A. J. Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo. J. Vis. Exp. (176), e62799, doi:10.3791/62799 (2021).