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In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
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JoVE Journal Nörobilim
In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

2,840 Views

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07:43 min

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November 20, 2021

DOI:

10.3791/62744-v

DOI:
10.3791/62744-v

•

07:43 min
November 20, 2021

•

5 Views
,
1DBT-National Brain Research Centre

Özet

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A protocol for imaging the dynamic microtubules in vivo using fluorescently labeled End binding protein has been presented. We described the methods to label, image, and analyze the dynamic microtubules in the Posterior lateral microtubule (PLM) neuron of C. elegans.

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Dey, S., Ghosh-Roy, A. In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons. J. Vis. Exp. (177), e62744, doi:10.3791/62744 (2021).

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