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Un metodo efficiente per l'induzione di cellule simili a epatociti diretti da cellule staminali embrionali umane
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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Un metodo efficiente per l'induzione di cellule simili a epatociti diretti da cellule staminali embrionali umane

2,601 Views

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08:05 min

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May 06, 2021

DOI:

10.3791/62654-v

DOI:
10.3791/62654-v

•

08:05 min
May 06, 2021

•

3 Views
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1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Özet

Automatically generated

Questo manoscritto descrive un protocollo dettagliato per la differenziazione delle cellule staminali embrionali umane (hESC) in cellule funzionali simili a epatociti (HHC) completando continuamente Activin A e CHIR99021 durante la differenziazione hESC in endoderma definitivo (DE).

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Cite this Article

Zhou, Q., Xie, X., Zhong, Z., Sun, P., Zhou, X. An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells. J. Vis. Exp. (171), e62654, doi:10.3791/62654 (2021).

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