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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法

2,601 Views

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08:05 min

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May 06, 2021

DOI:

10.3791/62654-v

DOI:
10.3791/62654-v

•

08:05 min
May 06, 2021

•

3 Views
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1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Özet

Automatically generated

本手稿描述了人类胚胎干细胞（hESC）分化成功能性肝细胞样细胞（HLCs）的详细协议，在hESC分化过程中连续补充Activin A和CHIR99021，以最终内分泌体（DE）。

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Cite this Article

Zhou, Q., Xie, X., Zhong, Z., Sun, P., Zhou, X. An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells. J. Vis. Exp. (171), e62654, doi:10.3791/62654 (2021).

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