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Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product
JoVE Journal
Kanser Araştırmaları
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JoVE Journal Kanser Araştırmaları
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

7,885 Views

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11:51 min

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September 22, 2021

DOI:

10.3791/62622-v

DOI:
10.3791/62622-v

•

11:51 min
September 22, 2021

•

45 Views
, , , , ,
1Cell Therapy Facility,Moffitt Cancer Center and Research Institute, 2Department of Immunology,Moffitt Cancer Center and Research Institute

Özet

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Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.

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Gamma-delta T CellsCytotoxic ActivityCancerAcute Myeloid LeukemiaExpansionEnrichmentApheresisCell TherapyElutriationCell CultureBioreactorZoledronic AcidIL-2Flow Cytometry

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Landin, A. M., Cox, C., Yu, B., Bejanyan, N., Davila, M., Kelley, L. Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product. J. Vis. Exp. (175), e62622, doi:10.3791/62622 (2021).

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