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使用 1-Cyano-4-二甲基亚胺四氟化物 （CDAP） 激活和结合可溶性聚糖
JoVE Journal
Ormancılık ve botanik
This content is Free Access.
JoVE Journal Ormancılık ve botanik
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
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使用 1-Cyano-4-二甲基亚胺四氟化物 （CDAP） 激活和结合可溶性聚糖

DOI:
10.3791/62597-v

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07:20 min

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June 14, 2021

•

,
1Fina Biosolutions LLC

'Bölümler'

  • 00:05Introduction
  • 01:18Polysaccharide Activation and Hydrazide Functionalization
  • 02:57Analysis of Hydrazide-Derivatized Polysaccharide
  • 05:45Results: Evaluation of Dextran, A Polysaccharide After CDAP Activation
  • 06:21Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

蛋白质和含胺配体可与氰化试剂、1-氰化物-4-二甲基甲基苯丙胺四氟溴酸酯（CDAP）激活的多糖共价相关，形成共价蛋白（利甘）-多糖结合体。本文描述了在 0 °C 和变 pH 值下执行受控 CDAP 激活以及执行激活多糖后续结合的改进协议。

'Etiketler'

Polysaccharide ActivationCDAPCyanogen BromideDerivatizationConjugationVaccinesDiagnostic ReagentsReductive AminationPH ControlAdipic DihydrazideDialysisHydrazide To Polysaccharide Ratio

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