The Differential Radial Capillary Action of Ligand Assay (DRaCALA) can be used to identify small ligand binding proteins of an organism by using an ORFeome library.
Schicketanz, M. L., Długosz, P., Zhang, Y. E. Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA). J. Vis. Exp. (169), e62331, doi:10.3791/62331 (2021).