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在体内成像完全活跃的脑组织在清醒斑马鱼幼虫和青少年由骷髅和皮肤去除
JoVE Journal
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JoVE Journal Nörobilim
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
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在体内成像完全活跃的脑组织在清醒斑马鱼幼虫和青少年由骷髅和皮肤去除

4,317 Views

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05:25 min

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February 10, 2021

DOI:

10.3791/62166-v

DOI:
10.3791/62166-v

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05:25 min
February 10, 2021

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7 Views
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1Cellular and Molecular Neurobiology, Zoological Institute,Technische Universität Braunschweig, 2Institute of Pathophysiology,University Medical Center of the Johannes Gutenberg University, 3Cell Physiology, Zoological institute,Technische Universität Braunschweig

Özet

Automatically generated

在这里，我们提出了一种方法，以图像斑马鱼胚胎大脑在体内到幼虫和幼年阶段。这种微创程序，根据电生理学方法改编，提供成熟神经元的细胞和亚细胞细节，并可与光遗传学和神经药理学研究相结合，以描述大脑功能和药物干预。

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Schramm, P., Hetsch, F., Meier, J. C., Köster, R. W. In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal. J. Vis. Exp. (168), e62166, doi:10.3791/62166 (2021).

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