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Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice
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Biyokimya
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JoVE Journal Biyokimya
Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice

DOI:
10.3791/62108-v

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06:10 min

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March 20, 2021

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, , , , ,
1Fuwai Hospital & Chinese Academy of Medical Sciences, State Key Laboratory of Cardiovascular Disease, Cardiovascular Institute, National Center for Cardiovascular Diseases,Peking Union Medical College, 2National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine,Fuwai Central-China Hospital, Central-China Subcenter of National Center for Cardiovascular Diseases

Bölümler

  • 00:04Introduction
  • 00:34Apical Resection Operation in Neonatal 1-day-old Cx3cr1GFP/+Mouse
  • 02:36Preparation of the Neonatal Cardiac Macrophage Suspension
  • 04:45Results: Immunofluorescence, Echocardiogram, Masons Staining, and Statistical Analysis of Neonatal Cardiac Macrophage Transplantation
  • 05:37Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We provide a protocol for neonatal cardiac macrophage separation and transplantation into an adult mouse heart, which could be a promising way to promote cardiac repair.

Etiketler

Neonatal MouseCardiac MacrophagesTransplantationAdult MiceCardiac RepairHeart RegenerationSurgical ProcedureApical ResectionCX3-CR1-GFPEnzyme Digestion

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