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Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
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Nörobilim
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JoVE Journal Nörobilim
Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions

Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions

DOI:
10.3791/61998-v

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08:04 min

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March 20, 2021

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, , , , ,
1Department of Genetics – Sorbonne Université, INSERM, CNRS,Institut de la Vision

Bölümler

  • 00:04Introduction
  • 00:58Recovery of the Chicken Embryos
  • 02:11Dissection of the Retinas
  • 02:50Preparation of Retinal Cell Suspension
  • 03:40Retinal Cell Seeding
  • 05:16Results: Epithelium-Derived Cone Viability Factor (EdCVF), Clone 0073-09-37
  • 07:21Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe a method to obtain primary cultures of cone photoreceptors from the retina of chicken embryos and its use for high content screening.

Etiketler

Cone ReceptorsChicken EmbryoRod-cone InteractionsRetinal DegenerationCone-enriched CulturePrimary Cell CultureHamburger And Hamilton StagesCarbon Dioxide-independent MediumRinger’s MediumTrypsin

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