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胎儿和成体神经干细胞衍生少突胶质细胞前体细胞培养物的高内涵筛选分化与成熟分析
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High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures
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胎儿和成体神经干细胞衍生少突胶质细胞前体细胞培养物的高内涵筛选分化与成熟分析

DOI:
10.3791/61988-v

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09:36 min

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March 10, 2021

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1Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR),University of Bologna

Bölümler

  • 00:04Introduction
  • 00:48Fetal Neural Stem Cell (NSC) Dissection and Isolation
  • 02:18Adult NSC Culture
  • 04:05Primary Neurosphere, Oligosphere, and Oligodendrocyte Precursor Cell (OPC) Differentiation
  • 05:05Inflammation-Mediated Differentiation Block and Oxygen-Glucose Deprivation (OGD) Cell Death Induction
  • 05:56High-Content Screening (HCS) Cell Viability, Lineage Composition, and Lineage-Specific Cell Death Analyses
  • 07:18Results: Representative Oligosphere Differentiation Characterization
  • 09:04Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们描述了星形胶质细胞和少突胶质细胞前体细胞的混合培养物的产生，这些细胞来自胎儿或成体神经干细胞，分化成成熟的少突胶质细胞，以及有毒刺激的体外建模。与基于细胞的高内涵筛选技术相结合，构建了一个可靠而强大的药物筛选系统。

Etiketler

Neural Stem CellsOligodendrocyte Precursor CellsAstrocytesFetalAdultHigh-content ScreeningDifferentiationMaturationMyelinationRemyelinationPathological ConditionsNon-enzymatic DissociationNeurosphere MediumCortexCorpus CallosumLateral Ventricles

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