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Neuroscience

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JoVE Journal Neuroscience
High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures
 

High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures

Article DOI: 10.3791/61988-v • 09:36 min • March 10th, 2021
March 10th, 2021 •
1
1Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna

Kapitel

Summary
Automatic Translation

We describe the production of mixed cultures of astrocytes and oligodendrocyte precursor cells derived from fetal or adult neural stem cells differentiating into mature oligodendrocytes, and in vitro modeling of noxious stimuli. The coupling with a cell-based high-content screening technique builds a reliable and robust drug screening system.

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Keywords: Neural Stem Cells Oligodendrocyte Precursor Cells Astrocytes Fetal Adult High-content Screening Differentiation Maturation Myelination Remyelination Pathological Conditions Non-enzymatic Dissociation Neurosphere Medium Cortex Corpus Callosum Lateral Ventricles
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