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使用纤维素凝块固定 的果蝇 组织用于实时成像
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging
Please note that all translations are automatically generated. Click here for the English version.

使用纤维素凝块固定 的果蝇 组织用于实时成像

2,764 Views

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08:52 min

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December 22, 2020

DOI:

10.3791/61954-v

DOI:
10.3791/61954-v

•

08:52 min
December 22, 2020

•

3 Views
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1Cell & Developmental Biology, School of Life Sciences,University of Dundee

Özet

Automatically generated

该协议描述了使用纤维蛋白块的样品固定应用，限制漂移，并允许在现场成像过程中添加和冲洗试剂。样品被转移到一滴含有培养基的纤维素中，然后通过添加血栓诱导聚合。

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Cite this Article

Januschke, J., Loyer, N. Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging. J. Vis. Exp. (166), e61954, doi:10.3791/61954 (2020).

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