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Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

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08:52 min

December 22, 2020

DOI:

08:52 min
December 22, 2020

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Summary

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This protocol describes applications of sample immobilization using Fibrin clots, limiting drifting, and allowing the addition and washout of reagents during live imaging. Samples are transferred to a drop of Fibrinogen containing culture medium on the surface of a coverslip, after which polymerization is induced by adding thrombin.

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