We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.
Omatsu-Kanbe, M., Fukunaga, R., Mi, X., Matsuura, H. An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice. J. Vis. Exp. (171), e61866, doi:10.3791/61866 (2021).