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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

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08:33 min

May 19, 2021

DOI:

08:33 min
May 19, 2021

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We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.

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