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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Pediatri Anabilim Dalı,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
Bölümler
- 00:04Introduction
- 00:51Testis Dissection and Preparation
- 02:10Transillumination-Assisted Microdissection
- 02:47Squash Preparation and Stage Verification
- 04:04Whole-Mount Seminiferous Tubule Immunostaining
- 07:04Results: Representative Mouse Seminiferous Epithelial Cycle Staging
- 09:16Conclusion
Dieses Protokoll beschreibt die transilluminationsunterstützte Mikrodissektion von Segmenten von erwachsenen Mausseminiferous Tubuli, die bestimmte Stadien des seminiferen Epithelzyklus darstellen, und Zelltypen darin und anschließende Immunostainierung von Squashpräparaten und intakten Tubulisegmenten.
