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Semi-quantitative Analyse von Peptidoglycan mittels Flüssigkeitschromatographie, Massenspektrometrie und Bioinformatik
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JoVE Journal Biyokimya
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Semi-quantitative Analyse von Peptidoglycan mittels Flüssigkeitschromatographie, Massenspektrometrie und Bioinformatik

3,776 Views

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09:09 min

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October 13, 2020

DOI:

10.3791/61799-v

DOI:
10.3791/61799-v

•

09:09 min
October 13, 2020

•

44 Views
, , ,
1Department of Molecular and Cellular Biology,University of Guelph, 2Mass Spectrometry Facility,University of Guelph

Özet

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Dieses Protokoll umfasst eine detaillierte Analyse der Peptidoglykanzusammensetzung mittels Flüssigchromatographie-Massenspektrometrie in Verbindung mit fortschrittlicher Merkmalsextraktion und bioinformatischer Analysesoftware.

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Anderson, E. M., Greenwood, N. A., Brewer, D., Khursigara, C. M. Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics. J. Vis. Exp. (164), e61799, doi:10.3791/61799 (2020).

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