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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Bioquímica
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JoVE Journal Bioquímica
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

3,782 Views

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09:09 min

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October 13, 2020

DOI:

10.3791/61799-v

DOI:
10.3791/61799-v

•

09:09 min
October 13, 2020

•

44 Views
, , ,
1Department of Molecular and Cellular Biology,University of Guelph, 2Mass Spectrometry Facility,University of Guelph

Summary

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This protocol covers a detailed analysis of peptidoglycan composition using liquid chromatography mass spectrometry coupled with advanced feature extraction and bioinformatic analysis software.

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PeptidoglycanMass SpectrometryBioinformaticsPeptidoglycomicsSacculi ExtractionSDS TreatmentEnzymatic DigestionLiquid Chromatography-mass Spectrometry

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Cite this Article

Anderson, E. M., Greenwood, N. A., Brewer, D., Khursigara, C. M. Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics. J. Vis. Exp. (164), e61799, doi:10.3791/61799 (2020).

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