Dergi
/
Biyokimya
/
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

DOI:
10.3791/61790-v

•

06:47 min

•

January 28, 2021

•

, , , ,
1Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 2School of Biotechnology and Health Sciences,Wuyi University

Bölümler

  • 00:05Introduction
  • 00:58Isolation of Plasma Lipoproteins
  • 03:00Results: Analysis of Lipoproteins and their Compositional Apolipoproteins Isolated from Rabbits on a Normal and High Cholesterol Diet
  • 05:32Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.

Etiketler

Plasma LipoproteinsUltracentrifugationLipoprotein FractionationHyperlipidemiaAtherosclerosisLipoprotein ProfileHigh Cholesterol DietHDLLDLVLDL

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Read Article