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4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

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08:38 min

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October 08, 2020

DOI:

10.3791/61736-v

DOI:
10.3791/61736-v

•

08:38 min
October 08, 2020

•

7 Views
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1CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain, 2IBIS (Instituto de Biomedicina de Sevilla),Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain

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Here, we present a protocol for preparing and mounting Caenorhabditis elegans embryos, recording development under a 4D microscope and tracing cell lineage.

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Escrich, V., Ezcurra, B., Gómez-Orte, E., Romero-Aranda, C., Miranda-Vizuete, A., Cabello, J. 4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy. J. Vis. Exp. (164), e61736, doi:10.3791/61736 (2020).

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