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腺病毒生产的有效方法
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
An Efficient Method for Adenovirus Production
Please note that all translations are automatically generated. Click here for the English version.

腺病毒生产的有效方法

11,607 Views

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10:06 min

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June 10, 2021

DOI:

10.3791/61691-v

DOI:
10.3791/61691-v

•

10:06 min
June 10, 2021

•

36 Views
, , ,
1Institute of Cellular Biology and Pathology “Nicolae Simionescu”

Özet

Automatically generated

在这里，我们提出了使用pAdEasy系统进行腺病毒生产的协议。该技术包括重新组合pAdTrack和pAdEasy-1质粒，腺病毒包装和放大，从细胞赖酸和培养基净化腺病毒颗粒，病毒滴定，和腺病毒的功能测试。

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Cite this Article

Dumitrescu, M., Trusca, V. G., Fenyo, I. M., Gafencu, A. V. An Efficient Method for Adenovirus Production. J. Vis. Exp. (172), e61691, doi:10.3791/61691 (2021).

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