Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
Bölümler
- 00:05Introduction
- 00:41Adeno-Associated Virus (AAV) Injection
- 02:23Gradient Refractive Index (GRIN) Lens Implantation
- 06:11GRIN Lens Implantation Validation
- 07:11Results: Representative GRIN Lens Implantation Validation and GCaMP Signal Evaluation
- 08:58Conclusion
In vivo microendoscopic calcium imaging is an invaluable tool that enables real-time monitoring of neuronal activities in freely behaving animals. However, applying this technique to the amygdala has been difficult. This protocol aims to provide a useful guideline for successfully targeting amygdala cells with a miniaturized microscope in mice.
