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単一ナノ粒子ダークフィールド顕微鏡を用いた細胞膜上の金ナノロッドの拡散ダイナミクスの可視化
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JoVE Journal Biyokimya
Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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単一ナノ粒子ダークフィールド顕微鏡を用いた細胞膜上の金ナノロッドの拡散ダイナミクスの可視化

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09:09 min

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March 05, 2021

DOI:

10.3791/61603-v

DOI:
10.3791/61603-v

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09:09 min
March 05, 2021

•

3 Views
, ,
1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education),Tsinghua University

Özet

Automatically generated

ここでは、従来の暗視野顕微鏡を用いて、細胞膜上の金ナノロッド(AuNRs)のダイナミクスを監視する方法を示す。単一の AuNR の位置と向きは ImageJ および MATLAB を使用して検出され、AuNRs の拡散状態は単一の粒子追跡解析によって特徴付けられる。

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Ge, F., Xue, J., He, Y. Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy. J. Vis. Exp. (169), e61603, doi:10.3791/61603 (2021).

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