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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

4,145 Views

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09:09 min

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March 05, 2021

DOI:

10.3791/61603-v

DOI:
10.3791/61603-v

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09:09 min
March 05, 2021

•

3 Views
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1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education),Tsinghua University

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Here, we show the use of traditional dark-field microscopy to monitor the dynamics of gold nanorods (AuNRs) on cell membrane. The location and orientation of single AuNRs are detected using ImageJ and MATLAB, and the diffusive states of AuNRs are characterized by single particle tracking analysis.

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Gold NanorodsSingle NanoparticleDarkfield MicroscopyCell MembraneDiffusion DynamicsTranslational DynamicsRotational DynamicsSingle Particle TrackingCTAB CoatingCell CultureImage ProcessingImageJ

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Ge, F., Xue, J., He, Y. Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy. J. Vis. Exp. (169), e61603, doi:10.3791/61603 (2021).

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