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Diffusional Dynamics of Gold Nanorods visualiseren op celmembraan met behulp van Single Nanoparticle Darkfield Microscopy
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JoVE Journal Biyokimya
Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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Diffusional Dynamics of Gold Nanorods visualiseren op celmembraan met behulp van Single Nanoparticle Darkfield Microscopy

4,143 Views

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09:09 min

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March 05, 2021

DOI:

10.3791/61603-v

DOI:
10.3791/61603-v

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09:09 min
March 05, 2021

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3 Views
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1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education),Tsinghua University

Özet

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Hier tonen we het gebruik van traditionele donkerveldmicroscopie om de dynamiek van gouden nanorods (AuNRs) op celmembraan te monitoren. De locatie en oriëntatie van enkele AuNR's worden gedetecteerd met behulp van ImageJ en MATLAB, en de diffusieve toestanden van AuNR's worden gekenmerkt door analyse van het volgen van afzonderlijke deeltjes.

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Ge, F., Xue, J., He, Y. Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy. J. Vis. Exp. (169), e61603, doi:10.3791/61603 (2021).

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