Dergi
/
Kimya
/
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
JoVE Journal
Kimya
This content is Free Access.
JoVE Journal Kimya
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

5,232 Views

•

09:24 min

•

December 18, 2020

DOI:

10.3791/61342-v

DOI:
10.3791/61342-v

•

09:24 min
December 18, 2020

•

2 Views
,
1Kimya Bölümü,Vanderbilt University, 2Vanderbilt Memory & Alzheimer’s Center,Vanderbilt University Medical Center, 3Vanderbilt Brain Institute,Vanderbilt University Medical Center, 4Vanderbilt Institute of Chemical Biology,Vanderbilt University Medical Center

Özet

Automatically generated

Combined precursor isotopic labeling and isobaric tagging (cPILOT) is an enhanced sample multiplexing strategy that is capable of increasing the number of samples that can be analyzed simultaneously with available isobaric tags. Incorporation of a robotic platform has greatly increased experimental throughput, reproducibility, and quantitative accuracy.

Explore More Videos

Automated CPILOTHigh ThroughputBiological InformationAgingAlzheimer’s DiseaseLiver HomogenateUreaDenaturation BufferDTTIAMCystine

Read Article

Cite this Article

Arul, A. B., Robinson, R. A. Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT). J. Vis. Exp. (166), e61342, doi:10.3791/61342 (2020).

Download .ris file

Copy

Share Video

.

Copy