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Establishment and Maintenance of Gnotobiotic American Cockroaches (Periplaneta americana)
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May 28, 2021
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This protocol is used in establishing and maintaining gnotobiotic American cockroaches (Periplaneta americana) by surface sterilizing the egg cases (oothecae) prior to hatching. These gnotobiotic insects contain their vertically transmitted Blattabacterium endosymbionts but have axenic guts.
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Dukes, H. E., Dyer, J. E., Ottesen, E. A. Establishment and Maintenance of Gnotobiotic American Cockroaches (Periplaneta americana). J. Vis. Exp. (171), e61316, doi:10.3791/61316 (2021).
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