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心房筋細胞、心室筋細胞、および非筋細胞の同時分離と培養
JoVE Journal
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JoVE Journal Biyoloji
Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
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心房筋細胞、心室筋細胞、および非筋細胞の同時分離と培養

6,831 Views

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11:53 min

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June 14, 2020

DOI:

10.3791/61224-v

DOI:
10.3791/61224-v

•

11:53 min
June 14, 2020

•

47 Views
, , , ,
1San Diego State University Heart Institute and the Department of Biology,San Diego State University

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単成マウス心臓の心房と心室の両方から筋細胞と非筋細胞を同時に分離する方法について説明する。このプロトコルは、高生存性心筋細胞および非筋細胞の一貫した収量をもたらし、フェノタイピングおよびインビトロ分析のための最適な細胞特異的培養条件を詳述する。

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Blackwood, E. A., Bilal, A. S., Azizi, K., Sarakki, A., Glembotski, C. C. Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart. J. Vis. Exp. (160), e61224, doi:10.3791/61224 (2020).

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