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Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
Bölümler
- 00:04Introduction
- 01:29Surgical Procedure
- 03:30Atrial Cell Isolation and Culture
- 05:46Ventricular Cell Isolation and Culture
- 07:46Stepwise Paradigm Calcium Reintroduction
- 09:04Results: Representative Adult Mouse Heart Atrial and Ventricular Myocyte and Non-Myocyte Characterization
- 11:09Conclusion
A method is described for the simultaneous isolation of myocytes and non-myocytes from both the atria and ventricles of a single adult mouse heart. This protocol results in consistent yields of highly viable cardiac myocytes and non-myocytes and details optimal cell-specific culture conditions for phenotyping and in vitro analysis.
