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来自成人鼠心脏的心外肌细胞、心室肌细胞和非肌细胞的同步隔离和文化
JoVE Journal
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Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
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来自成人鼠心脏的心外肌细胞、心室肌细胞和非肌细胞的同步隔离和文化

6,831 Views

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11:53 min

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June 14, 2020

DOI:

10.3791/61224-v

DOI:
10.3791/61224-v

•

11:53 min
June 14, 2020

•

47 Views
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1San Diego State University Heart Institute and the Department of Biology,San Diego State University

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一种方法被描述为同时从单个成年小鼠心脏的腹膜和心室中分离出肌细胞和非肌细胞。此协议可产生高度可行的心肌细胞和非肌细胞的一致产量，并详细说明表型和体外分析的最佳细胞特定培养条件。

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Cite this Article

Blackwood, E. A., Bilal, A. S., Azizi, K., Sarakki, A., Glembotski, C. C. Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart. J. Vis. Exp. (160), e61224, doi:10.3791/61224 (2020).

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