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分离中枢神经系统组织和相关脑膜，用于免疫细胞的下游分析
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
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分离中枢神经系统组织和相关脑膜，用于免疫细胞的下游分析

8,191 Views

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09:35 min

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May 19, 2020

DOI:

10.3791/61166-v

DOI:
10.3791/61166-v

•

09:35 min
May 19, 2020

•

33 Views
, , , , ,
1Department of Neurology, Geisel School of Medicine,Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine,Dartmouth College

Özet

Automatically generated

本文提出了两种优化的方案，用于检查中枢神经系统内的常驻和外周来源的免疫细胞，包括大脑、脊髓和脑膜。这些协议中的每一个都有助于确定在稳态和炎症条件下占据这些隔室的细胞的功能和组成。

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Cite this Article

DiSano, K. D., Linzey, M. R., Welsh, N. C., Meier, J. S., Pachner, A. R., Gilli, F. Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells. J. Vis. Exp. (159), e61166, doi:10.3791/61166 (2020).

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