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Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

8,228 Views

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09:35 min

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May 19, 2020

DOI:

10.3791/61166-v

DOI:
10.3791/61166-v

•

09:35 min
May 19, 2020

•

33 Views
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1Department of Neurology, Geisel School of Medicine,Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine,Dartmouth College

Summary

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This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.

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Cite this Article

DiSano, K. D., Linzey, M. R., Welsh, N. C., Meier, J. S., Pachner, A. R., Gilli, F. Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells. J. Vis. Exp. (159), e61166, doi:10.3791/61166 (2020).

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