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High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
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JoVE Journal Genetik
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

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08:19 min

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May 28, 2020

DOI:

10.3791/61132-v

DOI:
10.3791/61132-v

•

08:19 min
May 28, 2020

•

5 Views
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1Babraham Institute, 2Gurdon Institute,University of Cambridge, 3Genetik Bölümü,University of Cambridge, 4Wellcome Trust Genome Campus,Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease,The Rockefeller University

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In this article a high-throughput protocol for fast and reliable determination of gene expression levels in single or bulk C. elegans samples is described. This protocol does not require RNA isolation and produces cDNA directly from samples. It can be used together with high-throughput multiplexed nanofluidic real-time qPCR platforms.

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RT-qPCRC. ElegansNanofluidic TechnologyHigh-throughputSingle-sampleBulk-sampleRNA IsolationLysis BufferReverse TranscriptionPre-amplificationGene ExpressionInterindividual Variability

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Chauve, L., Le Pen, J., Hodge, F., Todtenhaupt, P., Biggins, L., Miska, E. A., Andrews, S., Casanueva, O. High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology. J. Vis. Exp. (159), e61132, doi:10.3791/61132 (2020).

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