Dergi
/
Gelişim Biyolojisi
/
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

5,093 Views

•

08:37 min

•

October 09, 2020

DOI:

10.3791/61086-v

DOI:
10.3791/61086-v

•

08:37 min
October 09, 2020

•

22 Views
,
1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center,Baylor Tıp Fakültesi

Özet

Automatically generated

We describe a technique for profiling microRNAs in early mouse embryos. This protocol overcomes the challenge of low cell input and small RNA enrichment. This assay can be used to analyze changes in miRNA expression over time in different cell lineages of the early mouse embryo.

Explore More Videos

Small RNA SequencingMicroRNAsEmbryonic DevelopmentLow Input SamplesEmbryo DissectionEmbryo StagingCell SuspensionCell SortingRNA Extraction

Read Article

Cite this Article

Keuls, R. A., Parchem, R. Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos. J. Vis. Exp. (164), e61086, doi:10.3791/61086 (2020).

Download .ris file

Copy

Share Video

.

Copy