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Production and Characterization of Human Macrophages from Pluripotent Stem Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

15,398 Views

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08:05 min

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April 16, 2020

DOI:

10.3791/61038-v

DOI:
10.3791/61038-v

•

08:05 min
April 16, 2020

•

40 Views
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1Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine,University of Edinburgh, 2Centre for Reproductive Health, The Queen’s Medical Research Institute,University of Edinburgh

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This protocol describes the robust generation of macrophages from human induced pluripotent stem cells, and methods for their subsequent characterization. Cell surface marker expression, gene expression, and functional assays are used to assess the phenotype and function of these iPSC-derived macrophages.

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Lopez-Yrigoyen, M., May, A., Ventura, T., Taylor, H., Fidanza, A., Cassetta, L., Pollard, J. W., Forrester, L. M. Production and Characterization of Human Macrophages from Pluripotent Stem Cells. J. Vis. Exp. (158), e61038, doi:10.3791/61038 (2020).

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