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单核细胞分离研究人类粘膜淋巴组织中先天免疫反应
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
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单核细胞分离研究人类粘膜淋巴组织中先天免疫反应

DOI:
10.3791/60914-v

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07:38 min

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June 14, 2020

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, , , ,
1Institute of Molecular Virology,Ulm University Medical Center, 2CNRS UMR-8601,Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy,Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department,Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin

Bölümler

  • 00:04Introduction
  • 00:44Dissection of Human Tonsillar Tissue
  • 01:58Isolation of Tonsillar Mononuclear Cells (TMCs)
  • 03:33Isolation of the TMCs by Cell Density Gradient
  • 04:42Preparing Cells for Use or Freezing
  • 05:14Results: Phenotype, Immunological Response, and Viability
  • 06:55Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在本协议中，我们解释了如何轻松地处理和培养来自健康人类的扁桃体单核细胞，从接受部分手术扁桃体切除术，以研究激活时的先天免疫反应，模仿粘膜组织中的病毒感染。

Etiketler

Tonsillar Mononuclear CellsMucosal ImmunityEx Vivo Immune ResponseCell IsolationCell StrainerDissectionPBSCell SuspensionCell Viability

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