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通过微分试穿素生成马赛克乳腺有机体
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Generation of Mosaic Mammary Organoids by Differential Trypsinization
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通过微分试穿素生成马赛克乳腺有机体

6,078 Views

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09:40 min

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March 11, 2020

DOI:

10.3791/60742-v

DOI:
10.3791/60742-v

•

09:40 min
March 11, 2020

•

4 Views
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1Department of Molecular, Cell and Developmental Biology,University of California, Santa Cruz

Özet

Automatically generated

乳腺是一种双层结构，包括外层皮和内光上皮细胞。提出是一种使用微分锥形制备器官的协议。这种有效的方法允许研究人员分别操作这两种细胞类型，以探索有关它们在乳腺形式和功能中的作用的问题。

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Cite this Article

Rubio, S., Cazares, O., Macias, H., Hinck, L. Generation of Mosaic Mammary Organoids by Differential Trypsinization. J. Vis. Exp. (157), e60742, doi:10.3791/60742 (2020).

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