Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Generation of Mosaic Mammary Organoids by Differential Trypsinization
6,090 Views
•
09:40 min
•
March 11, 2020
DOI:
Özet
Automatically generated
The mammary gland is a bilayered structure, comprising outer myoepithelial and inner luminal epithelial cells. Presented is a protocol to prepare organoids using differential trypsinization. This efficient method allows researchers to separately manipulate these two cell types to explore questions concerning their roles in mammary gland form and function.
Read Article
Cite this Article
Rubio, S., Cazares, O., Macias, H., Hinck, L. Generation of Mosaic Mammary Organoids by Differential Trypsinization. J. Vis. Exp. (157), e60742, doi:10.3791/60742 (2020).
Copy